Two iron-regulated cation transporters from tomato complement metal uptake-deficient yeast mutants

Plant Mol Biol. 2001 Mar;45(4):437-48. doi: 10.1023/a:1010620012803.

Abstract

Although iron deficiency poses severe nutritional problems to crop plants, to date iron transporters have only been characterized from the model plant Arabidopsis thaliana. To extend our molecular knowledge of Fe transport in crop plants, we have isolated two cDNAs (LeIRT1 and LeIRT2) from a library constructed from roots of iron-deficient tomato (Lycopersicon esculentum) plants, using the Arabidopsis iron transporter cDNA, IRTI, as a probe. Their deduced polypeptides display 64% and 62% identical amino acid residues to the IRT1 protein, respectively. Transcript level analyses revealed that both genes were predominantly expressed in roots. Transcription of LeIRT2 was unaffected by the iron status of the plant, while expression of LeIRT1 was strongly enhanced by iron limitation. The growth defect of an iron uptake-deficient yeast (Saccharomyces cerevisiae) mutant was complemented by LeIRT1 and LeIRT2 when ligated to a yeast expression plasmid. Transport assays revealed that iron uptake was restored in the transformed yeast cells. This uptake was temperature-dependent and saturable, and Fe2+ rather than Fe3+ was the preferred substrate. A number of divalent metal ions inhibited Fe2+ uptake when supplied at 100-fold or 10-fold excess. Manganese, zinc and copper uptake-deficient yeast mutants were also rescued by the two tomato cDNAs, suggesting that their gene products have a broad substrate range. The gene structure was determined by polymerase chain reaction experiments and, surprisingly, both genes are arranged in tandem with a tail-to-tail orientation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis Proteins*
  • Biological Transport / drug effects
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cation Transport Proteins*
  • Cations, Divalent / pharmacology
  • Cell Division / genetics
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Plant
  • Genetic Complementation Test
  • Iron / pharmacokinetics
  • Kinetics
  • Lycopersicon esculentum / genetics*
  • Lycopersicon esculentum / metabolism
  • Metals / pharmacokinetics*
  • Molecular Sequence Data
  • Mutation
  • Phenanthrolines / pharmacology
  • Phylogeny
  • Plant Proteins / genetics*
  • Protein Isoforms / genetics
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription, Genetic

Substances

  • Arabidopsis Proteins
  • Carrier Proteins
  • Cation Transport Proteins
  • Cations, Divalent
  • DNA, Complementary
  • IRT1 protein, Arabidopsis
  • Metals
  • Phenanthrolines
  • Plant Proteins
  • Protein Isoforms
  • RNA, Plant
  • bathophenanthroline disulfonic acid
  • Iron

Associated data

  • GENBANK/AF136579
  • GENBANK/AF136580
  • GENBANK/AF246266