Coup-TFII is up-regulated in adenovirus type 12 tumorigenic cells and is a repressor of MHC class I transcription

Virology. 2001 May 25;284(1):13-9. doi: 10.1006/viro.2001.0913.

Abstract

Down-regulation of the MHC class I enhancer in tumorigenic Ad12 cells is associated with strong binding of COUP-TF and negligible binding of activator NF-kappaB. By comparison, in nontumorigenic Ad5 cells, class I expression is high due to negligible binding of COUP-TF and strong binding of NF-kappaB. Here, we show that COUP-TFII, but not COUP-TFI, is expressed in Ad12-transformed cells. The dramatically stronger DNA binding of COUP-TFII to the class I enhancer in Ad12- compared to Ad5-transformed cells correlates with higher COUP-TFII promoter activity and higher levels of COUP-TFII mRNA and protein. Significantly, NF-kappaB p50/p52 double-knockout cells enabled us to demonstrate directly that COUP-TFII can completely repress both nonactivated and NF-kappaB-activated MHC class I transcription.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae*
  • Animals
  • Base Sequence
  • COUP Transcription Factor I
  • COUP Transcription Factor II
  • COUP Transcription Factors
  • Cell Cycle Proteins / metabolism
  • Cell Transformation, Neoplastic* / genetics
  • DNA / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology*
  • Electrophoresis, Polyacrylamide Gel
  • Genes, MHC Class I / genetics*
  • Mice
  • Molecular Sequence Data
  • NF-kappa B / metabolism
  • Promoter Regions, Genetic
  • RNA, Messenger / metabolism
  • Receptors, Steroid*
  • Repressor Proteins / physiology*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • Transcription, Genetic*
  • Tumor Cells, Cultured
  • Up-Regulation*

Substances

  • COUP Transcription Factor I
  • COUP Transcription Factor II
  • COUP Transcription Factors
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • NF-kappa B
  • Nr2f1 protein, mouse
  • Nr2f2 protein, mouse
  • RNA, Messenger
  • Receptors, Steroid
  • Repressor Proteins
  • Transcription Factors
  • protein p52, mammalian
  • DNA