The proliferative response of mouse jejunal crypt cells to radiation-induced cell depletion is not mediated exclusively by transforming growth factor alpha

Radiat Res. 2001 Jun;155(6):866-9. doi: 10.1667/0033-7587(2001)155[0866:tpromj]2.0.co;2.

Abstract

Several lines of correlative evidence link transforming growth factor alpha (Tgfa, also known as TGF-alpha) to proliferative activity in jejunal crypt cells. It is therefore tempting to hypothesize that, as a ligand of the epidermal growth factor, it mediates the compensatory proliferative burst in the crypts after radiation-induced cell killing. We have tested this hypothesis by comparing the repopulation response of wild-type and Tgfa-null mice, using the microcolony assay. Mice were exposed whole-body to (137)Cs gamma rays at a dose of approximately 1.6 Gy/min. Single doses and equal doses separated by 4 and 54 h were given. The rightward shift of the dose-response curves for 54 h was identical for wild-type and Tgfa-null mice, and there was no indication of a difference in radiosensitivity. This result indicates that Tgfa is not an essential component of the proliferative response of tissue to radiation-induced cell killing.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Division / radiation effects*
  • Dose-Response Relationship, Radiation
  • Jejunum / cytology
  • Jejunum / radiation effects*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Transforming Growth Factor alpha / genetics
  • Transforming Growth Factor alpha / physiology*

Substances

  • Transforming Growth Factor alpha