The Complete Genome Sequence of the Murine Respiratory Pathogen Mycoplasma Pulmonis

Nucleic Acids Res. 2001 May 15;29(10):2145-53. doi: 10.1093/nar/29.10.2145.

Abstract

Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology
  • Base Composition
  • Codon, Terminator / genetics
  • Computational Biology
  • Evolution, Molecular
  • Genetic Code
  • Genome*
  • Genomic Library
  • Humans
  • Internet
  • Lipoproteins / genetics
  • Mice
  • Molecular Sequence Data
  • Mutation / genetics
  • Mycoplasma / genetics*
  • Mycoplasma / immunology
  • Mycoplasma / pathogenicity*
  • Open Reading Frames / genetics
  • Polymorphism, Genetic / genetics
  • RNA, Bacterial / genetics
  • Recombination, Genetic / genetics
  • Repetitive Sequences, Nucleic Acid / genetics
  • Replication Origin / genetics
  • Respiratory System / microbiology*
  • Virulence / genetics

Substances

  • Antigens, Bacterial
  • Codon, Terminator
  • Lipoproteins
  • RNA, Bacterial

Associated data

  • GENBANK/AL445566