Impact of prednisone on TGF-beta1 and collagen in diaphragm muscle from mdx mice

Muscle Nerve. 2001 Mar;24(3):428-32. doi: 10.1002/1097-4598(200103)24:3<428::aid-mus1018>3.0.co;2-e.

Abstract

The purpose of this study was to assess the impact of prednisone treatment for 8 weeks on the level of transforming growth factor-beta 1 (TGF-beta1), hydroxyproline (HYP) concentrations, and level of the mature, nonreducible collagen cross-link hydroxylysylpyridinoline (HP) in diaphragm muscle from 12-week-old mdx mice. Diaphragm muscle from untreated mdx mice had a significantly higher level of TGF-beta1, HYP, and HP cross-link compared with normal C57BL/10J (control) mice. Prednisone treatment significantly reduced the level of TGF-beta1 and HYP in diaphragm from mdx mice to values similar to control mice, but resulted in a higher level of the HP cross-link compared with untreated mdx mice. These findings indicate that short-term treatment of mdx mice with prednisone can attenuate the fibrotic response in diaphragm muscle, possibly by mediating the level of TGF-beta. Although prednisone was beneficial in preventing collagen accumulation, it resulted in a higher level of the HP cross-link, presumably by decreasing collagen turnover

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / analysis*
  • Cross-Linking Reagents / analysis
  • Diaphragm / chemistry*
  • Diaphragm / drug effects
  • Glucocorticoids / pharmacology*
  • Hydroxyproline / analysis
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred mdx
  • Prednisone / pharmacology*
  • Transforming Growth Factor beta / analysis*
  • Transforming Growth Factor beta1

Substances

  • Cross-Linking Reagents
  • Glucocorticoids
  • Tgfb1 protein, mouse
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1
  • Collagen
  • Hydroxyproline
  • Prednisone