Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae

FEBS Lett. 2001 May 11;496(2-3):81-5. doi: 10.1016/s0014-5793(01)02412-7.

Abstract

In response to external amino acids, the permease-like sensor Ssy1 of Saccharomyces cerevisiae activates a pathway leading to transcriptional induction of several permease genes including AGP1 and PTR2. We previously reported that AGP1 induction requires Grr1, the F-box protein part of the SCF(Grr1) ubiquitin-ligase complex. We show here that ubiquitin, other components of SCF(Grr1) and the ubiquitin-conjugating enzyme Cdc34 are essential for AGP1 and PTR2 induction. This suggests that transduction to these genes of the amino acid signal generated by Ssy1 involves an SCF(Grr1)-catalysed ubiquitination step.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Transport Systems
  • Amino Acids / chemistry
  • Anaphase-Promoting Complex-Cyclosome
  • Carrier Proteins / biosynthesis
  • Dose-Response Relationship, Drug
  • F-Box Proteins
  • Fungal Proteins / metabolism*
  • Fungal Proteins / physiology
  • Gene Deletion
  • Genotype
  • Intracellular Signaling Peptides and Proteins
  • Ligases / physiology
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins / metabolism
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction*
  • Time Factors
  • Transcription, Genetic
  • Transduction, Genetic
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligase Complexes*
  • Ubiquitin-Protein Ligases
  • Ubiquitins / metabolism*
  • Ubiquitins / physiology
  • beta-Galactosidase / metabolism

Substances

  • Amino Acid Transport Systems
  • Amino Acids
  • Carrier Proteins
  • F-Box Proteins
  • Fungal Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • PTR2 protein, S cerevisiae
  • SSY1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Ubiquitins
  • GRR1 protein, S cerevisiae
  • CDC34 protein, S cerevisiae
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome
  • Ubiquitin-Protein Ligases
  • beta-Galactosidase
  • Ligases