Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer

Cancer Res. 2001 May 15;61(10):3882-5.


Caveolin-1 is an integral protein of caveolae, known to play important roles in signal transduction and lipid transport. We demonstrate that caveolin-1 expression is significantly increased in primary and metastatic human prostate cancer after androgen ablation therapy. We also show that caveolin-1 is secreted by androgen-insensitive prostate cancer cells, and that this secretion is regulated by steroid hormones. Significantly, caveolin-1 was detected in the MDL(3) fraction of serum specimens from patients with advanced prostate cancer and to a lesser extent in normal subjects. Conditioned media from high passage caveolin-1 secreting, androgen-insensitive, LNCaP cells stimulated increased viability and clonal growth of low passage, caveolin-1-negative, androgen-sensitive, LNCaP cells in vitro, and this effect was blocked by treating the media with caveolin-1 antibody. i.p. injections of caveolin-1 antibody suppressed the orthotopic growth and spontaneous metastasis of highly metastatic, androgen-insensitive caveolin-1-secreting mouse prostate cancer. Overall, our results establish caveolin-1 as an autocrine/paracrine factor that is associated with androgen-insensitive prostate cancer. We demonstrate the potential for caveolin-1 as a therapeutic target for this important malignancy.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Androgen Antagonists / pharmacology
  • Animals
  • Antibodies / pharmacology
  • Antineoplastic Agents, Hormonal / pharmacology
  • Bone Neoplasms / metabolism
  • Bone Neoplasms / pathology
  • Bone Neoplasms / secondary
  • Caveolin 1
  • Caveolins / antagonists & inhibitors
  • Caveolins / biosynthesis
  • Caveolins / metabolism*
  • Cell Division / physiology
  • Cell Survival / physiology
  • Culture Media, Conditioned
  • Humans
  • Male
  • Mice
  • Neoplasm Metastasis
  • Neoplasm Staging
  • Neoplasms, Hormone-Dependent / metabolism
  • Neoplasms, Hormone-Dependent / pathology
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology*


  • Androgen Antagonists
  • Antibodies
  • Antineoplastic Agents, Hormonal
  • CAV1 protein, human
  • Cav1 protein, mouse
  • Caveolin 1
  • Caveolins
  • Culture Media, Conditioned