Expression of a plant gene with sequence similarity to animal TGF-beta receptor interacting protein is regulated by brassinosteroids and required for normal plant development

Plant J. 2001 Apr;26(1):35-45. doi: 10.1046/j.1365-313x.2001.01007.x.

Abstract

Brassinosteroids (BRs) regulate the expression of numerous genes associated with plant development, and require the activity of a Ser/Thr receptor kinase to realize their effects. In animals, the transforming growth factor-beta (TGF-beta) family of peptides acts via Ser/Thr receptor kinases to have a major impact on several pathways involved in animal development and adult homeostasis. TGF-beta receptor-interacting protein (TRIP-1) was previously shown by others to be an intracellular substrate of the TGF-beta type II receptor kinase which plays an important role in TGF-beta signaling. TRIP-1 is a WD-repeat protein that also has a dual role as an essential subunit of the eukaryotic translation initiation factor eIF3 in animals, yeast and plants, thereby revealing a putative link between a developmental signaling pathway and the control of protein translation. In yeast, expression of a TRIP-1 homolog has also been closely associated with cell proliferation and progression through the cell cycle. We report here the novel observation that transcript levels of TRIP-1 homologs in plants are regulated by BR treatment under a variety of conditions, and that transgenic plants expressing antisense TRIP-1 RNA exhibit a broad range of developmental defects, including some that resemble the phenotype of BR-deficient and -insensitive mutants. This correlative evidence suggests that a WD-domain protein with reported dual functions in vertebrates and fungi might mediate some of the molecular mechanisms underlying the regulation of plant growth and development by BRs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antisense Elements (Genetics)
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism
  • Arabidopsis Proteins*
  • Blotting, Northern
  • Brassinosteroids
  • Cholestanols / metabolism*
  • Consensus Sequence
  • Eukaryotic Initiation Factor-3
  • Fabaceae / genetics*
  • Fabaceae / growth & development
  • Fabaceae / metabolism
  • Gene Expression Regulation, Plant
  • Molecular Sequence Data
  • Plant Growth Regulators / metabolism*
  • Plant Structures / metabolism
  • Plants, Genetically Modified
  • Plants, Medicinal*
  • Plants, Toxic*
  • Polymerase Chain Reaction
  • Protein Structure, Tertiary
  • Proteins / genetics*
  • Proteins / metabolism
  • Receptors, Transforming Growth Factor beta / metabolism*
  • Repetitive Sequences, Amino Acid
  • Sequence Homology, Amino Acid
  • Steroids, Heterocyclic / metabolism*
  • Tobacco / genetics*
  • Tobacco / growth & development
  • Tobacco / metabolism

Substances

  • Antisense Elements (Genetics)
  • Arabidopsis Proteins
  • Brassinosteroids
  • Cholestanols
  • Eukaryotic Initiation Factor-3
  • Plant Growth Regulators
  • Proteins
  • Receptors, Transforming Growth Factor beta
  • Steroids, Heterocyclic
  • TRIP-1 protein, Arabidopsis
  • EIF3I protein, human
  • brassinolide

Associated data

  • GENBANK/AF335551