Suppression of estrogen-dependent MMP-9 expression by Edpm5, a genetic locus for pituitary tumor growth in rat

Mol Cell Endocrinol. 2001 May 15;176(1-2):145-53. doi: 10.1016/s0303-7207(01)00401-4.


Chronic estrogen treatment results in elevated levels of the gelatinase pro-MMP-9 in the pituitary of tumor-susceptible Fischer 344 rats. In contrast, pituitary pro-MMP-9 level is not increased by estrogen treatment in rats of the tumor-resistant BN strain nor in rats of an F(1) hybrid of these strains. The gelatinase MMP-2 is also detected in rat pituitary, but its level is not affected by either estrogen or rat genotype. In 124 estrogen-treated rats from a backcross of the F(1) hybrid to the F344 strain, the levels of both monomer and dimer forms of pro-MMP-9 correlate with the tumor phenotypes mass, total DNA, and hemoglobin content of the pituitary. In this backcross, the QTL Edpm5 (_e_strogen-_d_ependent _p_ituitary _m_ass on Chromosome 5) has a significant effect on MMP-9 levels, with inheritance of the BN allele of Edpm5 correlating with suppression of estrogen-dependent MMP-9 expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Cell Division
  • Chromosome Mapping
  • Diethylstilbestrol / pharmacology
  • Enzyme Induction / drug effects
  • Estrogens, Non-Steroidal / pharmacology*
  • Female
  • Genetic Markers / genetics
  • Genotype
  • Matrix Metalloproteinase 9 / biosynthesis*
  • Matrix Metalloproteinase 9 / metabolism
  • Organ Specificity
  • Pituitary Neoplasms / enzymology
  • Pituitary Neoplasms / genetics*
  • Pituitary Neoplasms / pathology*
  • Quantitative Trait, Heritable
  • Rats
  • Rats, Inbred BN
  • Rats, Inbred F344
  • Uterus / drug effects
  • Uterus / enzymology
  • Uterus / metabolism


  • Estrogens, Non-Steroidal
  • Genetic Markers
  • Diethylstilbestrol
  • Matrix Metalloproteinase 9