Determination of UV-filters in sunscreens by HPLC

Fresenius J Anal Chem. 2001 Apr;369(7-8):638-41. doi: 10.1007/s002160100701.

Abstract

Simultaneous determination of six internationally authorised organic UV-filters in sunscreen formulations was performed by HPLC with UV spectrophotometric detection. The filters determined were: sulisobenzone, oxybenzone, octyl dimethyl PABA, octyl methoxycinnamate, octyl salicylate and homosalate. A C18 stationary phase and a mobile phase of ethanol water acetic acid (70:29.5:0.5) were used with a flow rate of 0.5 mL/min. UV measurements were carried out at 313 nm. The time required for the analysis was 25 min and the limits of detection were between 0.2 and 2 mg/L, except for sulisobenzone, which gave a limit of detection of 20 mg/L. The procedure proposed provides an accurate, fast and green analytical method, that does not involve toxic organic solvents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Indicators and Reagents
  • Sunscreening Agents / analysis
  • Sunscreening Agents / chemistry*
  • Ultraviolet Rays*

Substances

  • Indicators and Reagents
  • Sunscreening Agents