Analysis of the key pfcrt point mutation and in vitro and in vivo response to chloroquine in Yaoundé, Cameroon

J Infect Dis. 2001 Jun 15;183(12):1828-31. doi: 10.1086/320726. Epub 2001 May 16.


The putative key codon (Lys-76 in sensitive parasites and Thr-76 in resistant parasites) of the novel candidate gene for chloroquine resistance, Plasmodium falciparum chloroquine resistance transporter (pfcrt), was determined by polymerase chain reaction-restriction fragment length polymorphism from 111 Cameroonian isolates and was compared with in vivo and in vitro responses to chloroquine. The key codon was significantly associated (P< .001) with responses in vivo (92% sensitivity and 76% specificity) and in vitro (97% sensitivity and 81% specificity). Some discordant results were due to multiclonal infections. The high, but not perfect, correlation between the pfcrt polymorphism and the phenotype implies that a single point mutation in codon 76 of the pfcrt gene is the major, but possibly not the sole, determinant for chloroquine resistance.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Antimalarials / pharmacology
  • Cameroon
  • Child
  • Chloroquine / pharmacology*
  • Codon
  • Drug Resistance / genetics
  • Female
  • Humans
  • Male
  • Membrane Proteins / genetics*
  • Membrane Transport Proteins
  • Middle Aged
  • Phenotype
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / genetics*
  • Point Mutation*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Protozoan Proteins
  • Sensitivity and Specificity


  • Antimalarials
  • Codon
  • Membrane Proteins
  • Membrane Transport Proteins
  • PfCRT protein, Plasmodium falciparum
  • Protozoan Proteins
  • Chloroquine