Reactive oxygen species up-regulates cyclooxygenase-2, p53, and Bax mRNA expression in bovine luteal cells

Biochem Biophys Res Commun. 2001 Jun 1;284(1):203-10. doi: 10.1006/bbrc.2001.4927.

Abstract

Reactive oxygen species (ROS) are well-established modulators of luteal cell apoptosis in the estrous cycle. The objective of this study was to clarify the molecular mechanisms of luteolysis by characterizing the levels and regions of mRNAs involved in ROS-induced luteal cell apoptosis. Stimulation of bovine luteal cells by H2O2 resulted in the induction of apoptotic nuclear condensation and Caspase-3 activation. In addition, a marker for oxidative stress-damaged DNA, 8-hydroxy-2'-deoxyguanosine, was highly accumulated in the large luteal cells prepared from the late estrous stage. Reverse transcription polymerase chain reaction and Northern blot analysis demonstrated that mRNAs of cyclooxygenase (COX)-2, p53, and Bax were highly accumulated in the H2O2-treated cells. In situ hybridization revealed that these mRNAs were most abundantly expressed in the large luteal cells. These findings suggest that enhancement of ROS in the bovine corpus luteum induces expression of COX-2, p53, and Bax mRNAs, resulting in activation of the signaling pathway for luteal-cell apoptosis.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Animals
  • Apoptosis
  • Caspase 3
  • Caspases / metabolism
  • Cattle
  • Cells, Cultured
  • Corpus Luteum / cytology
  • Corpus Luteum / drug effects
  • Corpus Luteum / metabolism*
  • Cyclooxygenase 2
  • DNA / drug effects
  • DNA / metabolism
  • DNA Damage
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / metabolism
  • Female
  • Hydrogen Peroxide / metabolism
  • Hydrogen Peroxide / pharmacology
  • In Situ Hybridization
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Oxidative Stress / drug effects
  • Prostaglandin-Endoperoxide Synthases / genetics
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-bcl-2*
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / drug effects
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*
  • Up-Regulation / drug effects
  • bcl-2-Associated X Protein

Substances

  • Isoenzymes
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • Reactive Oxygen Species
  • Tumor Suppressor Protein p53
  • bcl-2-Associated X Protein
  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA
  • Hydrogen Peroxide
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Caspase 3
  • Caspases
  • Deoxyguanosine