Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein-DNA complexes with involvement of AML1/Runx-1/CBFalpha Runt domain, CBFbeta and the C/EBPbeta bZip region

Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):850-3. doi: 10.1107/s0907444901003900. Epub 2001 May 25.

Abstract

Three types of protein-DNA complexes, AML1/Runx-1/CBFalpha(Runt)-CBFbeta-C/EBPbeta(bZip)-DNA (CBFalpha-beta-C/EBPbeta-DNA), AML1/Runx-1/CBFalpha(Runt)-C/EBPbeta(bZip)-DNA (CBFalpha-C/EBPbeta-DNA) and AML1/Runx-1/CBFalpha(Runt)-DNA (CBFalpha-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C222(1), P2(1)2(1)2 and P2(1)2(1)2(1), respectively. The resolutions of CBFalpha-beta-C/EBPbeta-DNA and CBFalpha-C/EBPbeta-DNA crystals were both 3 A, while that of the CBFalpha-DNA crystal was 2.65 A. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFalpha-beta-C/EBPbeta-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFalpha-beta-C/EBPbeta-DNA.

MeSH terms

  • Animals
  • CCAAT-Enhancer-Binding Protein-beta / chemistry
  • Core Binding Factor Alpha 2 Subunit
  • Core Binding Factor alpha Subunits
  • Crystallization
  • Crystallography, X-Ray
  • DNA / chemistry*
  • DNA-Binding Proteins / chemistry
  • Mice
  • Neoplasm Proteins*
  • Nucleic Acid Conformation
  • Peptides / chemistry
  • Protein Conformation
  • Proto-Oncogene Proteins*
  • Recombinant Proteins / chemistry
  • Transcription Factors / chemistry

Substances

  • CCAAT-Enhancer-Binding Protein-beta
  • Core Binding Factor Alpha 2 Subunit
  • Core Binding Factor alpha Subunits
  • DNA-Binding Proteins
  • Neoplasm Proteins
  • Peptides
  • Proto-Oncogene Proteins
  • Recombinant Proteins
  • Runx1 protein, mouse
  • Transcription Factors
  • DNA