Crucial importance of PKC-beta(I) in LFA-1-mediated locomotion of activated T cells

Nat Immunol. 2001 Jun;2(6):508-14. doi: 10.1038/88700.


Crawling T cell locomotion in which activated lymphocyte function-associated antigen 1 (LFA-1) integrins participate is associated with translocation of the protein kinase C-beta (PKC-beta) isoenzyme to the microtubule cytoskeleton. In normal T cells and T lymphoma cell lines, this type of motility is accompanied by PKC-beta-sensitive cytoskeletal rearrangements and the formation of trailing cell extensions, which are supported by microtubules. Expression of PKC-beta(I) and enhanced green fluorescent protein (EGFP) in nonmotile PKC-beta-deficient T cells restored their locomotory behavior in response to a triggering stimulus delivered via LFA-1 and correlated directly with the degree of cell polarization. We have also shown that PKC-beta(I) is a component of the tubulin-enriched LFA-1-cytoskeletal complex assembled upon LFA-1 cross-linking. These observations may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Movement / physiology
  • Cell Polarity / immunology
  • Cell Polarity / physiology
  • Cytoskeleton / physiology
  • Green Fluorescent Proteins
  • Humans
  • In Vitro Techniques
  • Isoenzymes / genetics
  • Isoenzymes / physiology*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Lymphocyte Activation
  • Lymphocyte Function-Associated Antigen-1 / physiology*
  • Microtubules / physiology
  • Protein Kinase C / genetics
  • Protein Kinase C / physiology*
  • Protein Kinase C beta
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / physiology*


  • Isoenzymes
  • Luminescent Proteins
  • Lymphocyte Function-Associated Antigen-1
  • Recombinant Proteins
  • Green Fluorescent Proteins
  • Protein Kinase C
  • Protein Kinase C beta