Antifungal Resistance of Candidal Biofilms Formed on Denture Acrylic in Vitro

J Dent Res. 2001 Mar;80(3):903-8. doi: 10.1177/00220345010800031101.

Abstract

Denture biofilms represent a protective reservoir for oral microbes. The study of the biology of Candida in these biofilms requires a reliable model. A reproducible model of C. albicans denture biofilm was developed and used to determine the susceptibility of two clinically relevant C. albicans isolates against 4 antifungals. C. albicans, growing as a biofilm, exhibited resistance to amphotericin B, nystatin, chlorhexidine, and fluconazole, with 50% reduction in metabolic activity (50% RMA) at concentrations of 8, 16, 128, and > 64 microg/mL, respectively. In contrast, planktonically cultured C. albicans were susceptible (50% RMA for the same antifungals was obtained at 0.25, 1.0, 4.0, and 0.5 microg/mL, respectively). In conclusion, results obtained by means of our biofilm model show that biofilm-associated C. albicans cells, compared with cells grown in planktonic form, are resistant to antifungals used to treat denture stomatitis.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acrylic Resins*
  • Amphotericin B / administration & dosage
  • Amphotericin B / pharmacology
  • Anti-Infective Agents, Local / administration & dosage
  • Anti-Infective Agents, Local / pharmacology
  • Antifungal Agents / administration & dosage
  • Antifungal Agents / pharmacology*
  • Biofilms / drug effects*
  • Candida albicans / classification
  • Candida albicans / drug effects*
  • Candidiasis, Oral / drug therapy
  • Candidiasis, Oral / microbiology
  • Chlorhexidine / administration & dosage
  • Chlorhexidine / pharmacology
  • Colony Count, Microbial
  • Denture Bases / microbiology*
  • Dose-Response Relationship, Drug
  • Drug Resistance, Microbial
  • Fluconazole / administration & dosage
  • Fluconazole / pharmacology
  • Galactose / pharmacology
  • Glucose / pharmacology
  • Humans
  • Indicators and Reagents
  • Nystatin / administration & dosage
  • Nystatin / pharmacology
  • Polymethyl Methacrylate
  • Saliva / microbiology
  • Statistics as Topic
  • Stomatitis, Denture / drug therapy
  • Stomatitis, Denture / microbiology
  • Sucrose / pharmacology
  • Tetrazolium Salts
  • Time Factors

Substances

  • Acrylic Resins
  • Anti-Infective Agents, Local
  • Antifungal Agents
  • Indicators and Reagents
  • Tetrazolium Salts
  • 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide
  • Nystatin
  • Sucrose
  • Amphotericin B
  • Fluconazole
  • Polymethyl Methacrylate
  • Glucose
  • Chlorhexidine
  • Galactose