Single nucleotide polymorphisms in intron 2 of the human interleukin-1 receptor antagonist (IL-1Ra) gene: further definition of the IL-1 beta and IL-1Ra polymorphisms in North American Caucasians and Taiwanese Chinese

Tissue Antigens. 2001 Apr;57(4):318-24. doi: 10.1034/j.1399-0039.2001.057004318.x.

Abstract

Previous studies have suggested that a variable number tandem repeat (VNTR) polymorphism in the second intron of the interleukin-1 receptor antagonist (IL-1Ra) gene and the single nucleotide polymorphisms at positions -511 and +3954 of the IL-1beta gene might be associated with increased risks of chronic inflammatory diseases, autoimmune diseases and gastric cancer. In the present study, IL-1beta and IL-1Ra genotypes were analyzed among Asians in Taiwan and Caucasians in North America. We identified a novel polymorphism with 3 nucleotide substitutions in the IL-1Ra VNTR 2-repeat allele. One of the substitutions corresponds with the fourth 3' end nucleotide of the reverse primer that is often used for analysis of the IL-1Ra-associated VNTR locus. Mismatching between this primer and the 2-repeat allele can cause misleading amplification results when stringent conditions are used for annealing. The estimated haplotype frequencies of the variant IL-1 genes were significantly different between Taiwanese and Caucasians. The frequency of the pro-inflammatory IL-1Ra 2-repeat allele was significantly lower in Taiwanese than in Caucasians. In contrast, the frequencies of the pro-inflammatory IL-1beta -511T allele and +3954C allele were significantly higher among Taiwanese compared with Caucasians.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Asian Continental Ancestry Group / genetics*
  • Base Pair Mismatch / genetics
  • DNA Primers / genetics
  • European Continental Ancestry Group / genetics*
  • Gene Frequency / immunology
  • Genotype
  • Haplotypes / immunology
  • Histocompatibility Testing
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / genetics*
  • Introns / genetics*
  • Introns / immunology
  • Minisatellite Repeats / genetics
  • North America
  • Polymorphism, Single Nucleotide* / immunology
  • Receptors, Interleukin-1 / antagonists & inhibitors*
  • Sialoglycoproteins / genetics*
  • Taiwan

Substances

  • DNA Primers
  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Receptors, Interleukin-1
  • Sialoglycoproteins