Abstract
Antisense oligonucleotides provide a promising approach to investigating gene function in vivo, but their ability to offer unambiguous insights into phenotypes has been debated. The recent use of morpholino antisense oligonucleotides in zebrafish embryos may prove a major advance, but rigorous controls are essential.
Publication types
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Gene Expression Regulation, Developmental*
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Morpholines / pharmacology*
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Oligonucleotides, Antisense / pharmacology*
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RNA, Messenger / metabolism
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Sea Urchins / embryology
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Sea Urchins / genetics
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Zebrafish / embryology
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Zebrafish / genetics
Substances
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Morpholines
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Oligonucleotides, Antisense
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RNA, Messenger