Morpholino antisense oligonucleotides: tools for investigating vertebrate development

Genome Biol. 2001;2(5):REVIEWS1015. doi: 10.1186/gb-2001-2-5-reviews1015. Epub 2001 Apr 26.


Antisense oligonucleotides provide a promising approach to investigating gene function in vivo, but their ability to offer unambiguous insights into phenotypes has been debated. The recent use of morpholino antisense oligonucleotides in zebrafish embryos may prove a major advance, but rigorous controls are essential.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Gene Expression Regulation, Developmental*
  • Morpholines / pharmacology*
  • Oligonucleotides, Antisense / pharmacology*
  • RNA, Messenger / metabolism
  • Sea Urchins / embryology
  • Sea Urchins / genetics
  • Zebrafish / embryology
  • Zebrafish / genetics


  • Morpholines
  • Oligonucleotides, Antisense
  • RNA, Messenger