Antagonism of ORLI receptor produces an algesic effect in the rat formalin test

Neuroreport. 2001 May 25;12(7):1323-7. doi: 10.1097/00001756-200105250-00007.

Abstract

The authors investigated the role of endogenously released nociceptin (also known as orphanin FQ) spinal and supraspinal nociceptive transmission during the rat formalin test by examining the effect of intrathecal and intracerebroventricular injection of J-113397, a non-peptidyl ORL1 receptor selective antagonist. When J-113397 was injected intrathecally or intracerebroventricularly 10 min before the formalin injection, it enhanced the agitation behavior induced by paw formalin injection. This suggested that paw formalin injection induced nociceptin release in the spinal cord and the supraspinal brain sites, that this endogenously released nociceptin produced an analgesic effect and that J-113397 antagonized this analgesic effect of nociceptin and produced an algesic effect in the rat formalin test.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzimidazoles / pharmacology
  • Brain / drug effects
  • Brain / metabolism
  • Dose-Response Relationship, Drug
  • Efferent Pathways / drug effects
  • Efferent Pathways / metabolism
  • Injections, Intraventricular
  • Injections, Spinal
  • Male
  • Motor Activity / drug effects
  • Motor Activity / physiology
  • Narcotic Antagonists* / pharmacology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Nociceptors / drug effects
  • Nociceptors / metabolism*
  • Opioid Peptides / metabolism*
  • Pain / chemically induced
  • Pain / metabolism*
  • Pain / physiopathology
  • Pain Measurement / drug effects*
  • Piperidines / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Opioid / metabolism
  • Spinal Cord / drug effects
  • Spinal Cord / metabolism*

Substances

  • Benzimidazoles
  • J 113397
  • Narcotic Antagonists
  • Opioid Peptides
  • Piperidines
  • Receptors, Opioid
  • nociceptin
  • nociceptin receptor