Studying protein dynamics in living cells

Nat Rev Mol Cell Biol. 2001 Jun;2(6):444-56. doi: 10.1038/35073068.


Since the advent of the green fluorescent protein, the subcellular localization, mobility, transport routes and binding interactions of proteins can be studied in living cells. Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components. Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biological Transport
  • Green Fluorescent Proteins
  • Humans
  • Image Processing, Computer-Assisted
  • Luminescent Proteins / metabolism
  • Proteins / metabolism*


  • Luminescent Proteins
  • Proteins
  • Green Fluorescent Proteins