Differential regulation of the NO3- and NH4+ transporter genes AtNrt2.1 and AtAmt1.1 in Arabidopsis: relation with long-distance and local controls by N status of the plant

Plant J. 2001 Apr;26(2):143-55. doi: 10.1046/j.1365-313x.2001.01016.x.

Abstract

Regulation of root N uptake by whole-plant signalling of N status was investigated at the molecular level in Arabidopsis thaliana plants through expression analysis of AtNrt2.1 and AtAmt1.1. These two genes encode starvation-induced high-affinity NO3- and NH4+ transporters, respectively. Split-root experiments indicate that AtNrt2.1 expression is controlled by shoot-to-root signals of N demand. Together with 15NO3- influx, the steady-state transcript level of this gene is increased in NO3--fed roots in response to N deprivation of another portion of the root system. Thus AtNrt2.1 is the first identified molecular target of the long-distance signalling informing the roots of the whole plant's N status. In contrast, AtAmt1.1 expression is predominantly dependent on the local N status of the roots, as it is mostly stimulated in the portion of the root system directly experiencing N starvation. The same behaviour was found for NH4+ influx, suggesting that the NH4+ uptake system is much less efficient than the NO3- uptake system, to compensate for a spatial restriction of N availability. Other major differences were found between the regulations of AtNrt2.1 and AtAmt1.1 expression. AtNrt2.1 is strongly upregulated by moderate level of N limitation, while AtAmt1.1 transcript level is markedly increased only under severe N deficiency. Unlike AtNrt2.1, AtAmt1.1 expression is not stimulated in a nitrate reductase-deficient mutant after transfer to NO3- as sole N source, indicating that NO3- per se acts as a signal repressing transcription of AtAmt1.1. These results reveal two fundamentally different types of mechanism involved in the feedback regulation of root N acquisition by the N status of the plant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anion Transport Proteins*
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins*
  • Biomass
  • Blotting, Northern
  • Carrier Proteins / genetics*
  • Cation Transport Proteins*
  • Gene Expression Regulation, Plant*
  • Isotope Labeling
  • Nitrates / metabolism*
  • Nitrogen / metabolism
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Roots
  • Quaternary Ammonium Compounds / metabolism*
  • RNA, Messenger
  • RNA, Plant
  • Signal Transduction

Substances

  • Anion Transport Proteins
  • Arabidopsis Proteins
  • Carrier Proteins
  • Cation Transport Proteins
  • NRT2 protein, Arabidopsis
  • Nitrates
  • Plant Proteins
  • Quaternary Ammonium Compounds
  • RNA, Messenger
  • RNA, Plant
  • ammonium transporters, plant
  • nitrate transporters
  • Nitrogen