A highly specific pathogen-responsive promoter element from the immediate-early activated CMPG1 gene in Petroselinum crispum

Plant J. 2001 Apr;26(2):217-27. doi: 10.1046/j.1365-313x.2001.01015.x.

Abstract

Within the complex signalling network from pathogen-derived elicitor perception to defense-related gene activation, some immediate-early responding genes may have pivotal roles in downstream transcriptional regulation. We have identified the parsley (Petroselinum crispum) ELI17 gene as a particularly fast-responding gene possessing a new type of W box-containing, elicitor-responsive promoter element, E17. Highly selective E17-mediated reporter gene expression at pathogen infection sites in transgenic Arabidopsis thaliana plants demonstrated the potential of this promoter element for designing new strategies in resistance breeding as well as for further analysis of the early components of defense-related gene activation mechanisms. The protein encoded by the ELI17 gene exhibits various structural characteristics of established transcription factors and is designated as a CMPG protein according to the first four strictly conserved amino acids defining a newly emerging class of plant-specific proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algal Proteins / genetics
  • Amino Acid Sequence
  • Apiaceae / genetics*
  • Base Sequence
  • Cytidine Monophosphate / classification
  • Cytidine Monophosphate / genetics
  • DNA-Binding Proteins / genetics
  • Fungal Proteins
  • Genes, Plant / genetics
  • Host-Parasite Interactions
  • Molecular Sequence Data
  • Oomycetes / physiology
  • Plant Proteins / biosynthesis*
  • Plant Proteins / genetics
  • Plants, Genetically Modified
  • Promoter Regions, Genetic* / genetics
  • Proteins
  • RNA, Messenger / metabolism
  • RNA, Plant / biosynthesis
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Rhizoctonia / physiology
  • Signal Transduction
  • Species Specificity
  • Transcription Factors / genetics
  • Transcriptional Activation

Substances

  • Algal Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Plant Proteins
  • Proteins
  • RNA, Messenger
  • RNA, Plant
  • Transcription Factors
  • elicitin, Phytophthora
  • pathogenesis-related proteins, plant
  • Cytidine Monophosphate

Associated data

  • GENBANK/AF274563
  • GENBANK/AF274564
  • GENBANK/AF274565