The Drosophila Su(var)2-10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family

Genes Dev. 2001 Jun 1;15(11):1334-48. doi: 10.1101/gad.877901.

Abstract

The conserved heterochromatic location of centromeres in higher eukaryotes suggests that intrinsic properties of heterochromatin are important for chromosome inheritance. Based on this hypothesis, mutations in Drosophila melanogaster that alter heterochromatin-induced gene silencing were tested for effects on chromosome inheritance. Here we describe the characterization of the Su(var)2-10 locus, initially identified as a Suppressor of Position-Effect Variegation. Su(var)2-10 is required for viability, and mutations cause both minichromosome and endogenous chromosome inheritance defects. Mitotic chromosomes are improperly condensed in mutants, and polytene chromosomes are structurally abnormal and disorganized in the nucleus. Su(var)2-10 encodes a member of the PIAS protein family, a group of highly conserved proteins that control diverse functions. SU(VAR)2-10 proteins colocalize with nuclear lamin in interphase, and little to no SU(VAR)2-10 is found on condensed mitotic chromosomes. SU(VAR)2-10 is present at some polytene chromosome telomeres, and FISH analyses in mutant polytene nuclei revealed defects in telomere clustering and telomere-nuclear-lamina associations. We propose that Su(var2-10 controls multiple aspects of chromosome structure and function by establishing/maintaining chromosome organization in interphase nuclei.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Chromatin / genetics*
  • Chromatin / metabolism
  • Chromatin / ultrastructure
  • Chromosome Structures / genetics*
  • Chromosome Structures / metabolism
  • Chromosome Structures / ultrastructure
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Drosophila / cytology
  • Drosophila / genetics*
  • Drosophila / metabolism
  • Fluorescent Antibody Technique
  • Gene Silencing
  • In Situ Hybridization, Fluorescence
  • Kruppel-Like Transcription Factors
  • Mitosis / genetics
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Polymerase Chain Reaction
  • Protein Inhibitors of Activated STAT
  • Proteins / genetics*
  • Proteins / metabolism
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Telomere / genetics
  • Telomere / metabolism
  • Telomere / ultrastructure
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Ubiquitin-Protein Ligases

Substances

  • Carrier Proteins
  • Chromatin
  • DNA-Binding Proteins
  • Kruppel-Like Transcription Factors
  • Nuclear Proteins
  • Protein Inhibitors of Activated STAT
  • Proteins
  • Repressor Proteins
  • Transcription Factors
  • ZBTB17 protein, human
  • Miz1 protein, mouse
  • Ubiquitin-Protein Ligases