High-performance liquid chromatographic assay of zonisamide in human plasma using a non-porous silica column

J Chromatogr B Biomed Sci Appl. 2001 May 5;755(1-2):337-41. doi: 10.1016/s0378-4347(01)00062-7.


A new method for measuring zonisamide (ZNS) in plasma by high-performance liquid chromatography was developed by using a 2-microm reversed-phase non-porous silica column. ZNS in plasma was first purified with a column extraction technique and injected onto the non-porous silica column. Calibration curve was linear over the concentration range of 1-80 microg/ml in plasma. The recoveries of ZNS added to plasma were more than 95.4% with the coefficient of variation less than 9.0%. We developed a rapid routine method using the non-porous silica column that was accurate and improved solvent consumption in the measurement of ZNS.

MeSH terms

  • Anticonvulsants / blood*
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Isoxazoles / blood*
  • Isoxazoles / pharmacokinetics
  • Silicon Dioxide*
  • Solvents
  • Zonisamide


  • Anticonvulsants
  • Isoxazoles
  • Solvents
  • Zonisamide
  • Silicon Dioxide