Structure and expression of the human histamine H4-receptor gene

Biochem Biophys Res Commun. 2001 Jun 8;284(2):301-9. doi: 10.1006/bbrc.2001.4976.


We report the characterization by genomics-based approach of the human H4-receptor gene structure. The H4-receptor gene have been mapped by radiation hybrid experiments (Gene Bridge 4) on chromosome 18q11.2, between the AFMBB11WH5 and CHLC.GATA85D10 markers. The H4-receptor gene spans more than 21 kbp and contains three exons separated by two large introns (>7 kbp). RT-PCR analysis showed that the H4-receptor gene encoded a 3.7 kb mRNA which did not seem to be alternatively spliced within its coding region. The H4-receptor transcripts were found to be highly expressed in peripheral tissues implicated in inflammatory responses such as leukocytes, spleen, lung, and liver. In addition, low expression level of the H4-receptor mRNA was also detected in several human brain regions. Analysis of the 5'-flanking region of the H4-receptor gene did not reveal the existence of canonical TATA or CAAT-box. However, several putative regulatory elements mediating TNFalpha or IL-6-stimulated transcriptional activation were detected. The uteroglobin promoter binding factor, known to mediate anti-inflammatory response of uteroglobin, in the lung, was also found in this region. Thus, the description of the H4-receptor gene promoter region will facilitate the elucidation of its transcriptional control by factors secreted during inflammatory responses.

MeSH terms

  • 3' Untranslated Regions / genetics
  • 5' Untranslated Regions / genetics
  • Base Sequence
  • Blotting, Southern
  • Brain / metabolism
  • Genomic Library
  • Humans
  • Leukocytes / metabolism
  • Liver / metabolism
  • Lung / metabolism
  • Molecular Sequence Data
  • Organ Specificity
  • Physical Chromosome Mapping*
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Radiation Hybrid Mapping
  • Receptors, G-Protein-Coupled*
  • Receptors, Histamine / genetics*
  • Receptors, Histamine H4
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Spleen / metabolism


  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • HRH4 protein, human
  • RNA, Messenger
  • Receptors, G-Protein-Coupled
  • Receptors, Histamine
  • Receptors, Histamine H4