Up-regulation of calcineurin Abeta mRNA in the Alzheimer's disease brain: assessment by cDNA microarray

Biochem Biophys Res Commun. 2001 Jun 8;284(2):310-6. doi: 10.1006/bbrc.2001.4968.


Recent advances in cDNA microarray technology have made it possible to analyze expression of more than 8000 genes. Using this technology, gene expression in the hippocampus containing neurofibrillary tangle-associated lesions from an Alzheimer's disease (AD) patient was compared with expression in the parietal cortex from the same patient that lacked these lesions. We also compared gene expression using a control brain. The top 20 named genes significantly up-regulated or down-regulated only in the AD brain were determined. The most up-regulated gene proved to be calcineurin Abeta mRNA (CAbeta). In situ hybridization histochemistry revealed that CAbeta was significantly up-regulated in pyramidal neurons of the hippocampus in the AD brain. RT-PCR analysis revealed that CAbeta was up-regulated in the hippocampus from two out of three AD brains while there were no changes in three control brains. Our study suggests that CAbeta may play a crucial role in the pathophysiological mechanisms in AD.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / metabolism*
  • Alzheimer Disease / pathology
  • Brain / metabolism*
  • Brain / pathology
  • Calcineurin / genetics
  • Calcineurin / metabolism*
  • Female
  • Gene Expression Profiling
  • Hippocampus / metabolism
  • Hippocampus / pathology
  • Histocytochemistry
  • Humans
  • In Situ Hybridization
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Male
  • Oligonucleotide Array Sequence Analysis*
  • Parietal Lobe / metabolism
  • Parietal Lobe / pathology
  • RNA, Messenger / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation*


  • Isoenzymes
  • RNA, Messenger
  • Calcineurin