The central cannabinoid receptor inactivation suppresses endocrine reproductive functions

Biochem Biophys Res Commun. 2001 Jun 8;284(2):363-8. doi: 10.1006/bbrc.2001.4977.

Abstract

The function of central cannabinoid (CB1) receptor was investigated in the regulation of the pituitary-gonad axis in CB1 receptor knockout male mouse. Serum luteinizing hormone (LH) and testosterone (T) levels and basal T secretion in vitro of testes were significantly decreased in mutant (CB1-/-) mice. The receptor agonist, anandamide (ANA), suppressed LH and T secretion in wild type (CB1+/+) mice but had no effect in receptor inactivated animals. The results are the first descriptions indicating the direct action of CB1 receptors on LH and T secretion and the immunohistological demonstration of CB1 receptors in the Leydig cells. The results also indicate that CB1 receptors are responsible for the effects of exogenous cannabinoids on reproductive functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acids / pharmacology
  • Endocannabinoids
  • Endocrine System / drug effects
  • Endocrine System / metabolism*
  • Immunohistochemistry
  • In Vitro Techniques
  • Leydig Cells / cytology
  • Leydig Cells / metabolism
  • Luteinizing Hormone / blood
  • Luteinizing Hormone / metabolism
  • Male
  • Mice
  • Mice, Knockout
  • Organ Size / drug effects
  • Piperidines / pharmacology
  • Pituitary Gland, Anterior / cytology
  • Pituitary Gland, Anterior / drug effects
  • Pituitary Gland, Anterior / metabolism
  • Polyunsaturated Alkamides
  • Pyrazoles / pharmacology
  • Receptors, Cannabinoid
  • Receptors, Drug / antagonists & inhibitors
  • Receptors, Drug / deficiency*
  • Receptors, Drug / genetics
  • Receptors, Drug / metabolism
  • Rimonabant
  • Testis / drug effects
  • Testis / metabolism*
  • Testosterone / blood
  • Testosterone / metabolism

Substances

  • Arachidonic Acids
  • Endocannabinoids
  • Piperidines
  • Polyunsaturated Alkamides
  • Pyrazoles
  • Receptors, Cannabinoid
  • Receptors, Drug
  • Testosterone
  • Luteinizing Hormone
  • Rimonabant
  • anandamide