The Insulin-Sensitive GLUT4 Storage Compartment Is a Postendocytic and Heterogeneous Population Recruited by Acute Exercise

Biochem Biophys Res Commun. 2001 Jun 8;284(2):490-5. doi: 10.1006/bbrc.2001.4983.

Abstract

Insulin and acute exercise stimulate glucose transport in skeletal muscle by translocating GLUT4 glucose transporters to the cell surface. GLUT4 is distributed in skeletal muscle in two intracellular membrane populations, an endosomal pool that remains unaltered after insulin treatment and an storage population that is markedly GLUT4 depleted in response to insulin. Here we have further characterized the storage GLUT4 compartment in regard to protein composition and sensitivity to acute exercise. This GLUT4 compartment contained IRAP (insulin-regulated aminopeptidase), transferrin receptors or mannose-6-phosphate/IGF-II receptors, indicating a postendocytic origin. Insulin administration caused a depletion of GLUT4 and IRAP but no changes in transferrin receptors, which suggests that this pool is heterogeneous. In addition, acute exercise caused a marked GLUT4 depletion in the storage compartment, whereas no changes were detected in the endosomal population. In all, our data indicate that the GLUT4 storage population represents a postendocytic and heterogeneous compartment; the storage compartment represents the recruitment site that triggers GLUT4 translocation to the cell surface in response to both insulin and acute exercise.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminopeptidases / metabolism
  • Animals
  • Cell Compartmentation / drug effects
  • Cell Compartmentation / physiology*
  • Cystinyl Aminopeptidase
  • Endosomes / metabolism
  • Glucose Transporter Type 4
  • Immunoblotting
  • Insulin / metabolism*
  • Insulin / pharmacology
  • Male
  • Monosaccharide Transport Proteins / metabolism*
  • Muscle Proteins*
  • Muscle, Skeletal / drug effects
  • Muscle, Skeletal / metabolism*
  • Physical Exertion / physiology*
  • Protein Transport / physiology
  • Rats
  • Rats, Wistar
  • Receptor, IGF Type 2 / metabolism
  • Receptors, Transferrin / metabolism
  • Subcellular Fractions / metabolism

Substances

  • Glucose Transporter Type 4
  • Insulin
  • Monosaccharide Transport Proteins
  • Muscle Proteins
  • Receptor, IGF Type 2
  • Receptors, Transferrin
  • Slc2a4 protein, rat
  • Aminopeptidases
  • Cystinyl Aminopeptidase
  • leucyl-cystinyl aminopeptidase