Expression and possible role of cyclin D3 in human pancreatic adenocarcinoma

Anticancer Res. Mar-Apr 2001;21(2A):1043-8.


Background: Cyclin D3, one of the D type cyclins, has been known as a cell cycle modulator at the G1-S phase. In the present study, we investigated its expression in pancreatic adenocarcinoma in order to elucidate its clinical role.

Materials and methods: We examined cyclin D3 as well as, cyclin D1 overexpression in 60 pancreatic adenocarcinoma by means of immunohistochemistry.

Results: Cyclin D3 overexpression was found in 33.3% (20 cases) of the cases, being more frequently seen in cases showing high Ki-67 labeling index, stage IV, moderate- or poor-differentiation, lymph node metastasis and large size and cyclin D1 overexpression. Multivariate logistic analysis independently linked cyclin D3 overexpression to tumor size (p = 0.0392) and stage (p = 0.0312). Cyclin D1 overexpression was observed in 41.7% (25 cases) and was related to Ki-67 labeling index, cyclin A overexpression, stage, carcinoma differentiation, lymph node metastasis, tumor size, lymphatic invasion, perineural invasion and retropancreatic invasion. It was independently linked to carcinoma differentiation (p = 0.0081), lymph node metastasis (p = 0.0426) and lymphatic invasion (p = 0.0269).

Conclusion: These results suggest that cyclin D3, as well as cyclin D1, plays a role in the progression, including cell proliferating activity, of pancreatic adenocarcinoma.

MeSH terms

  • Adenocarcinoma / pathology
  • Adenocarcinoma / physiopathology*
  • Adult
  • Aged
  • Cyclin D1 / biosynthesis
  • Cyclin D1 / physiology
  • Cyclin D3
  • Cyclins / biosynthesis
  • Cyclins / physiology*
  • Disease Progression
  • Female
  • Humans
  • Ki-67 Antigen / biosynthesis
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Neoplasm Invasiveness
  • Pancreatic Neoplasms / pathology
  • Pancreatic Neoplasms / physiopathology*


  • CCND3 protein, human
  • Cyclin D3
  • Cyclins
  • Ki-67 Antigen
  • Cyclin D1