Calretinin promoter for suicide gene expression in malignant mesothelioma

Anticancer Res. Mar-Apr 2001;21(2A):1111-4.

Abstract

To achieve specific and efficient expression of transfected suicide gene in malignant mesothelioma cells, we explored the 5-flanking region (2.2 kb) of the calretinin gene as a tumor-specific promoter. RT-PCR analysis demonstrated the expression of calretinin mRNA in two human malignant mesothelioma cell lines (H2052 and 211 H). A reporting vector containing the calretinin promoter (pCAL-LUC) exhibited relatively high luciferase activity in these cell lines. After transfecting an expression vector containing the calretinin promoter-bound thymidine kinase gene (pCAL-TK) into the cells, we examined their sensitivity to ganciclovir (GCV). In the mesothelioma cell lines, the pCAL-TK transfected cells became about 100 times more sensitive to GCV than the parental cells. The calretinin promoter seems to be a promising candidate as a specific and efficient promoter in malignant mesothelioma.

MeSH terms

  • Antiviral Agents / pharmacology
  • Calbindin 2
  • Ganciclovir / pharmacology
  • Gene Expression
  • Genetic Therapy / methods*
  • Humans
  • Mesothelioma / genetics*
  • Mesothelioma / therapy
  • Promoter Regions, Genetic*
  • S100 Calcium Binding Protein G / genetics*
  • Simplexvirus / enzymology*
  • Thymidine Kinase / genetics*
  • Tumor Cells, Cultured

Substances

  • Antiviral Agents
  • CALB2 protein, human
  • Calbindin 2
  • S100 Calcium Binding Protein G
  • Thymidine Kinase
  • Ganciclovir