A hematopoietic-specific transmembrane protein, Art-1, is possibly regulated by AML1

Biochem Biophys Res Commun. 2001 Jun 15;284(3):714-22. doi: 10.1006/bbrc.2001.5005.

Abstract

The functions of AML1 in hematopoietic differentiation are repressed by AML1-mutants including the AML1/ETO chimeric protein, which is seen in t(8;21) acute myeloid leukemia. Erythroid progenitors of the patients with t(8;21) AML expressed AML1/ETO. To investigate the effect of AML1/ETO in erythroid cells, we made a tetracycline-regulated AML1/ETO overexpression system in mouse erythroleukemic (MEL) cells. Enforced AML1/ETO repressed the terminal erythroid differentiation. Furthermore, we performed representational difference analysis using this MEL cell system to clone the downstream targets of AML1 in erythroid cell differentiation. We cloned a novel transmembrane protein, Art-1 (AML1-regulated transmembrane protein 1), which is a member of tetramembrane spanning superfamily. Art-1 expression was restricted in hematopoietic cells. It was upregulated by AML1 and downregulated by AML1/ETO in both erythroid and myeloid cells, and increased during erythroid cell differentiation. Art-1 may play an important role in the differentiation of erythroid cells, possibly as a direct downstream target of AML1.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Differentiation
  • Cloning, Molecular
  • Core Binding Factor Alpha 2 Subunit
  • DNA-Binding Proteins / physiology*
  • Enzyme Inhibitors / pharmacology
  • Erythrocytes / metabolism*
  • Erythrocytes / physiology
  • Hydroxamic Acids / pharmacology
  • Leukemia, Erythroblastic, Acute
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Membrane Proteins / physiology
  • Mice
  • Molecular Sequence Data
  • Myeloid Cells / metabolism
  • Oncogene Proteins, Fusion / biosynthesis
  • Oncogene Proteins, Fusion / genetics
  • Proto-Oncogene Proteins*
  • RNA, Messenger / biosynthesis
  • RUNX1 Translocation Partner 1 Protein
  • Tetracycline / pharmacology
  • Tissue Distribution
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Tumor Cells, Cultured

Substances

  • AML1-ETO fusion protein, human
  • Core Binding Factor Alpha 2 Subunit
  • DNA-Binding Proteins
  • Enzyme Inhibitors
  • Hydroxamic Acids
  • Membrane Proteins
  • Oncogene Proteins, Fusion
  • Phemx protein, mouse
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • RUNX1 Translocation Partner 1 Protein
  • Runx1 protein, mouse
  • Transcription Factors
  • trichostatin A
  • Tetracycline

Associated data

  • GENBANK/AF291425