Evaluation of an optical immunoassay for the rapid detection of influenza A and B viral antigens

Eur J Clin Microbiol Infect Dis. 2001 Apr;20(4):280-3. doi: 10.1007/s100960100475.


An optical immunoassay for the rapid detection of influenza types A and B viral antigens, FLU OIA (Biostar, USA), was prospectively compared with antigen detection methods and cell culture on 400 respiratory specimens during an influenza outbreak that occurred in Switzerland in 1998/1999. The FLU OIA had an overall sensitivity of 64.4% (95%CI, 56.3-71.7%) and a specificity of 94.9% (95%CI, 89.8-97.7%). Using specimens from pediatric and adolescent patients, the sensitivity obtained (71.8%; 95%CI, 61.7-80%) was different than that achieved with specimens from adult patients (51.4%; 95%CI, 36.5-65%) (P=0.004). The results show that rapid diagnostic tests with higher sensitivity and specificity for the detection of influenza virus are needed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Viral / analysis*
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Immunoassay
  • Infant
  • Infant, Newborn
  • Influenza A virus / immunology*
  • Influenza B virus / immunology*
  • Male
  • Middle Aged
  • Prospective Studies
  • Sensitivity and Specificity


  • Antigens, Viral