Expression and Induction of Collagenases (MMP-8 and -13) in Plasma Cells Associated With Bone-Destructive Lesions

J Pathol. 2001 Jun;194(2):217-24. doi: 10.1002/path.854.


Matrix metalloproteinases (MMPs) collectively degrade extracellular matrix and basement membrane proteins in chronic inflammation and bone-destructive lesions. This study examined the ability of immunoglobulin-producing plasma cells, typically present in sites of chronic inflammation, to express collagenases (MMP-8 and -13) in vivo and in vitro. Phorbol-12-myristate-13-acetate, interleukin-6, and tumour necrosis factor-alpha and heparin with the tumour promoter or cytokines potently enhanced (up to nine-fold) MMP-8 and -13 expression by the RPMI 8226 myeloma cell line, as evidenced by western blotting and semi-quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemical analysis and in situ hybridization revealed that plasma cells expressed MMP-8 and -13 focally in periapical granulomas, odontogenic cysts, and malignant plasmacytomas. MMP-8 and MMP-13 from plasma cells can participate in bone organic matrix destruction at sites of chronic inflammation and neoplastic growth. Since MMP-13 was more frequently expressed than MMP-8 in plasma cells of strongly recurring keratocysts and malignant plasmacytomas, it is concluded that plasma cell MMP-13 has a particularly important role in benign and malignant bone-destructive lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Diseases / enzymology*
  • Bone Neoplasms / enzymology
  • Chi-Square Distribution
  • Collagenases / analysis
  • Collagenases / genetics
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • In Situ Hybridization
  • Matrix Metalloproteinase 13
  • Matrix Metalloproteinase 8 / analysis
  • Matrix Metalloproteinase 8 / genetics
  • Matrix Metalloproteinases / analysis*
  • Multiple Myeloma / enzymology
  • Odontogenic Cysts / enzymology
  • Periapical Granuloma / enzymology
  • Plasma Cells / enzymology*
  • Plasmacytoma / enzymology
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured / enzymology


  • RNA, Messenger
  • Collagenases
  • MMP13 protein, human
  • Matrix Metalloproteinase 13
  • Matrix Metalloproteinases
  • Matrix Metalloproteinase 8