The incorporation of wrong bases by DNA polymerase I following gamma-irradiation of DNA-like templates

Biochim Biophys Acta. 1974 Apr 27;349(1):23-31. doi: 10.1016/0005-2787(74)90004-5.


The synthesis of polydeoxyribose polymers by Escherichia coli DNA polymerase I has been investigated with control and gamma-irradiated DNA-like polymer templates containing only two bases. The results show that irradiation of a poly(dA) strand leads to the incorporation of dG, whereas irradiation of poly(dC) and poly(dG) strands both lead to the incorporation of dA. Irradiation of poly(dT) does not lead to the incorporation of any wrong base. The wrong bases are incorporated into the complementary strand of the newly synthesised DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • DNA Damage*
  • DNA Polymerase I / chemistry
  • DNA Polymerase I / metabolism*
  • DNA, Complementary / chemistry
  • Dose-Response Relationship, Radiation
  • Escherichia coli
  • Gamma Rays
  • Poly A / chemistry
  • Polydeoxyribonucleotides / chemistry
  • Polydeoxyribonucleotides / radiation effects
  • Templates, Genetic*


  • DNA, Complementary
  • Polydeoxyribonucleotides
  • poly(d(A-T))2
  • poly(dT)-2poly(dA)
  • Poly A
  • poly(dA)
  • poly(dG).poly(dC)
  • DNA Polymerase I