Modulation of TcR/CD3-zeta chain expression by a circulating factor derived from ovarian cancer patients

Br J Cancer. 2001 Jun 15;84(12):1624-9. doi: 10.1054/bjoc.2001.1847.


In women with ovarian cancer, suppression of components of the immune system may promote tumour development. Previous studies in ovarian cancer have demonstrated that decreased expression and function of the T-cell receptor (TcR)-associated signal transducing zeta-chain correlates with deficient immune responsiveness of T cells. In this study, sera and ascitic fluids obtained from woman with advanced ovarian cancer were found to suppress the expression of TcR-associated zeta chain. This suppression of zeta chain expression was dose-dependent and was not observed with biologic fluids obtained from healthy women. The factor responsible for the loss of zeta chain was purified from ascites and characterized as a protein with an appropriate molecular weight of 14 kD. Suppression of T-cell TcR-zeta was specific, since neither lck nor ZAP-70 expression was affected, while zeta chain was almost completely suppressed. This selective suppression of TcR-zeta expression by the 14 kD ascites-derived factor was shown to operate at the mRNA level. By defining the mechanism through which this protein modulates TcR-zeta chain levels, it might be possible to ultimately prevent the suppressive influences of the tumour microenvironment and restor immune competence in patients with ovarian carcinoma.

MeSH terms

  • Adult
  • Ascites
  • Blotting, Western
  • Carcinoma / genetics
  • Carcinoma / pathology*
  • Case-Control Studies
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunocompetence
  • Immunosuppression*
  • Membrane Proteins / biosynthesis*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / pathology*
  • Proteins / chemistry
  • Proteins / isolation & purification
  • Proteins / pharmacology
  • Receptor-CD3 Complex, Antigen, T-Cell / biosynthesis*
  • Receptors, Antigen, T-Cell / biosynthesis*


  • Membrane Proteins
  • Proteins
  • Receptor-CD3 Complex, Antigen, T-Cell
  • Receptors, Antigen, T-Cell
  • antigen T cell receptor, zeta chain