Genetic Characterization of a Chlamydophila Pneumoniae Isolate From an African Frog and Comparison to Currently Accepted Biovars

Syst Appl Microbiol. 2001 Apr;24(1):63-6. doi: 10.1078/0723-2020-00016.

Abstract

The amphibian isolate DE177 identified as Chlamydophila (C.) pneumoniae was sequenced in five genomic regions: 16S ribosomal RNA gene, 16-23S intergenic spacer, ompA, ompB, and groESL genes. Comparison with corresponding sequences of the currently accepted equine, human and koala biovars of C. pneumoniae revealed that koala strains represented the most closely related taxon, although sequence dissimilarities in the ompA (VD4) and ompB gene regions were noted. In this respect, the present isolate is distinct from a previously described frog isolate (Berger et al., 1999) whose sequence analysis yielded identity to the koala biovar. As three of the nucleotide substitutions in ompA (VD4) of DE177 will be translated into two altered amino acids the possible existence of another biovar is discussed.

Publication types

  • Case Reports

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Proteins / genetics
  • Central African Republic
  • Chaperonins / genetics
  • Chlamydophila Infections / veterinary*
  • Chlamydophila pneumoniae / genetics*
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal Spacer / genetics
  • Female
  • Male
  • Molecular Sequence Data
  • RNA, Ribosomal, 16S / genetics
  • Ranidae / microbiology*
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer
  • GroESL protein, Bacteria
  • RNA, Ribosomal, 16S
  • Chaperonins

Associated data

  • GENBANK/AF347606
  • GENBANK/AF347607
  • GENBANK/AF347608
  • GENBANK/AF347610
  • PIR/AF347609