Heat shock protein 27 was up-regulated in cisplatin resistant human ovarian tumor cell line and associated with the cisplatin resistance

Cancer Lett. 2001 Jul 26;168(2):173-81. doi: 10.1016/s0304-3835(01)00532-8.


To understand the molecular basis for failure of cisplatin (CDDP) based chemotherapy, we compared gene expressions between CDDP sensitive and resistant ovarian tumor cell line, 2008 and 2008/C13*5.25, by mRNA differential display. We detected both up-regulated and down-regulated bands in the resistant cell and found some of them to be positive on Northern blotting. DNA sequencing revealed one to be mitochondrial heat shock protein 75. We found that HSP27 and HSP70 were also up-regulated in the resistant cell by Western blotting. Further, transient transfection with the HSP27 sense gene made the sensitive cell more resistant, while transient transfection with the antisense gene made it more sensitive.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Blotting, Western
  • Cisplatin / pharmacology*
  • Cloning, Molecular
  • DNA, Antisense / genetics
  • DNA, Complementary / genetics
  • Drug Resistance, Neoplasm / genetics
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / drug effects
  • HSP70 Heat-Shock Proteins / biosynthesis
  • HSP70 Heat-Shock Proteins / genetics
  • HSP90 Heat-Shock Proteins*
  • Heat-Shock Proteins / biosynthesis*
  • Heat-Shock Proteins / genetics
  • Humans
  • Mitochondria / metabolism
  • Ovarian Neoplasms / drug therapy*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / metabolism*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Transfection
  • Tumor Cells, Cultured
  • Up-Regulation / drug effects


  • Antineoplastic Agents
  • DNA, Antisense
  • DNA, Complementary
  • HSP70 Heat-Shock Proteins
  • HSP90 Heat-Shock Proteins
  • Heat-Shock Proteins
  • RNA, Messenger
  • TRAP1 protein, human
  • Cisplatin