Orphanin FQ/nociceptin binds to functionally coupled ORL1 receptors on human immune cell lines and alters peripheral blood mononuclear cell proliferation

Brain Res Bull. 2001 Apr;54(6):655-60. doi: 10.1016/s0361-9230(01)00482-8.

Abstract

Orphanin FQ/nociceptin (OFQ/N) has been shown to modulate nociception, responses to stress and anxiety. We investigated OFQ/N function in human immune cells. We find that monocytic U937, T lymphocytic CEM, and MOLT-4 cell lines express OFQ/N binding sites at levels comparable to that of human SH-SY5Y neuroblastoma cells. We show that OFQ/N receptors are functionally coupled to G proteins in these cells. Finally OFQ/N decreases proliferation of phytohemagglutinin-stimulated peripheral blood mononuclear cells in vitro at doses ranging from 10(-13) to 10(-8) M. Thus, our data suggest that OFQ/N and OFQ/N receptor may act as an immunomodulatory system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Binding Sites / drug effects
  • Binding Sites / physiology
  • CHO Cells / drug effects
  • CHO Cells / metabolism
  • Cell Division / drug effects
  • Cell Division / physiology
  • Cell Line / cytology
  • Cell Line / drug effects
  • Cell Line / metabolism*
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cricetinae
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacokinetics
  • Humans
  • Immune System / cytology
  • Immune System / drug effects
  • Immune System / metabolism*
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / metabolism*
  • Neuroblastoma
  • Opioid Peptides / immunology
  • Opioid Peptides / metabolism*
  • Opioid Peptides / pharmacology
  • Phytohemagglutinins / pharmacology
  • Radioligand Assay
  • Receptors, Opioid / drug effects
  • Receptors, Opioid / immunology
  • Receptors, Opioid / metabolism*
  • Sulfur Radioisotopes / pharmacology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism

Substances

  • Opioid Peptides
  • Phytohemagglutinins
  • Receptors, Opioid
  • Sulfur Radioisotopes
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • nociceptin
  • nociceptin receptor