Heterogeneous nuclear ribonucleoprotein-H plays a suppressive role in visceral myogenesis

Mech Dev. 2001 Jun;104(1-2):79-87. doi: 10.1016/s0925-4773(01)00377-x.


Mouse embryonic mesenchymal cells undergo spontaneous smooth muscle (SM) differentiation upon spreading/elongation in culture (Relan et al., J. Cell Biol. 147 (1999) 1341; Yang et al., Development 125 (1998) 2621; Yang et al., Development 126 (1999) 3027). Using these cells we generated a subtracted cDNA library to identify potential suppressors of SM myogenesis. One of the differentially expressed genes was heterogeneous nuclear ribonucleoprotein-H (hnRNP-H), which is involved in pre-mRNA alternative splicing. hnRNP-H was highly expressed in mesenchymal cells prior to the onset of SM differentiation, but its expression rapidly decreased in mesenchymal cells undergoing SM myogenesis. In vivo, the drop in hnRNP-H expression was restricted to visceral SM cells. Antisense oligodeoxynucleotide and antisense RNA were used to inhibit hnRNP-H synthesis in SM-differentiating mesenchymal cells and in embryonic lung explants. A decrease in hnRNP-H levels resulted in upregulation of SM-specific gene expression and increased bronchial SM development in lung explants. hnRNP-H overexpression in cell cultures had the opposite effect. These studies, therefore, indicate a novel role for hnRNP-H in the control of visceral myogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Bronchi / embryology
  • Cell Differentiation
  • Epithelial Cells / metabolism
  • Gene Library
  • Heterogeneous-Nuclear Ribonucleoprotein Group F-H
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Immunohistochemistry
  • Lung / embryology
  • Lung / pathology
  • Mesoderm / metabolism
  • Mice
  • Mice, Inbred ICR
  • Muscle, Smooth / embryology*
  • Oligonucleotides, Antisense / metabolism
  • Organ Culture Techniques
  • RNA, Messenger / metabolism
  • Ribonucleoproteins / physiology*
  • Time Factors
  • Tissue Distribution
  • Transfection


  • Heterogeneous-Nuclear Ribonucleoprotein Group F-H
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Oligonucleotides, Antisense
  • RNA, Messenger
  • Ribonucleoproteins