The Identification of Microorganisms by Fluorescence in Situ Hybridisation

Curr Opin Biotechnol. 2001 Jun;12(3):231-6. doi: 10.1016/s0958-1669(00)00204-4.

Abstract

Fluorescence in situ hybridisation (FISH) with rRNA-targeted oligonucleotide probes facilitates the rapid and specific identification of individual microbial cells in their natural environments. Over the past year there have been a number of methodological developments in this area and new applications of FISH in microbial ecology and biotechnology have been reported.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Environmental Microbiology
  • Geologic Sediments / chemistry
  • Geologic Sediments / microbiology
  • In Situ Hybridization, Fluorescence / methods*
  • Molecular Probes / chemistry
  • RNA, Ribosomal, 16S / genetics
  • RNA, Ribosomal, 16S / physiology*
  • Sensitivity and Specificity
  • Sewage / microbiology
  • Symbiosis

Substances

  • Molecular Probes
  • RNA, Ribosomal, 16S
  • Sewage