Sequence comparisons among dispersed members of the Brassica S multigene family in an S9 genome

Mol Genet Genomics. 2001 May;265(3):526-34. doi: 10.1007/s004380000441.

Abstract

Self-incompatibility (SI) systems prevent self-pollination and promote outbreeding. In Brassica, the SI genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are members of the S multigene family, which share the SLG-like domain (S domain), which encodes a putative receptor. We have cloned members of the S multigene family from the S9 haplotype of B. campestris (syn. rapa). In addition, eight distinct genomic regions harboring 10 SLG/SRK-like genes were characterized in the present study. Sequence analysis revealed two novel SRK-like genes, BcRK3 and BcRK6 (for B. campestris receptor kinases 3 and 6, respectively). Other genes that were characterized included SFR2 (for S gene family receptor 2), SLR2 (for S locus related gene 2), and a pseudogene. Based on phylogenetic analysis of the nucleotide sequences of the S domain regions, SLG and SRK appear to be distinct from other members of the S multigene family. Linkage analysis showed that most members of the S multigene family are dispersed in the Brassica genome, and that SLR1 (S locus related gene 1) is not linked to the SLR2 in B. campestris.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Brassica / genetics*
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Evolution, Molecular
  • Genetic Linkage
  • Genetic Variation
  • Genome, Plant*
  • Haplotypes
  • Homozygote
  • Models, Genetic
  • Multigene Family
  • Phylogeny
  • Polymorphism, Restriction Fragment Length
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA

Substances

  • DNA, Complementary