FebA: a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum

Biochim Biophys Acta. 2001 May 28;1519(1-2):65-9. doi: 10.1016/s0167-4781(01)00219-6.


We have identified a gene encoding a eukaryotic initiation factor 4E-binding protein (4E-BP) in the EST database of the Dictyostelium cDNA project. The Dictyostelium 4E-BP, designated febA (four e-binding), showed significant similarity to mammalian 4E-BPs. Northern blot analysis revealed that febA was expressed at a high level in the vegetative growth phase but the level of expression decreased during late development. The gene was shown to be non-essential since disruption of the gene had no severe effect; the null mutant proliferated normally and formed normal fruiting bodies. However, strains overexpressing the gene could not be established, suggesting that an excess of FebA protein may have a lethal effect on the cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / genetics*
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • Dictyostelium / genetics*
  • Genetic Vectors
  • Molecular Sequence Data
  • Phosphoproteins*
  • Sequence Alignment


  • Carrier Proteins
  • DNA, Complementary
  • Phosphoproteins