Cloning and characterization of the major groESL operon from a nitrogen-fixing cyanobacterium Anabaena sp. strain L-31

H Rajaram; A D Ballal; S K Apte; T Wiegert; W Schumann

doi:10.1016/s0167-4781(01)00222-6

Cloning and characterization of the major groESL operon from a nitrogen-fixing cyanobacterium Anabaena sp. strain L-31

Biochim Biophys Acta. 2001 May 28;1519(1-2):143-6. doi: 10.1016/s0167-4781(01)00222-6.

Authors

H Rajaram¹, A D Ballal, S K Apte, T Wiegert, W Schumann

Affiliation

¹ Cell Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.

PMID: 11406285
DOI: 10.1016/s0167-4781(01)00222-6

Abstract

The major heat-shock-responsive operon groESL has been cloned from the cyanobacterial diazotroph Anabaena. The bicistronic operon harbors an upstream negative regulatory CIRCE element and is transcriptionally activated upon temperature upshift. The deduced amino acid sequence displays strong identity/similarity with other cyanobacterial GroES and GroEL proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anabaena / chemistry
Anabaena / genetics*
Bacterial Proteins / chemistry
Bacterial Proteins / genetics*
Base Sequence
Chaperonins / chemistry
Chaperonins / genetics*
Cloning, Molecular
DNA, Complementary / chemistry
DNA, Complementary / isolation & purification
Genes, Bacterial*
Molecular Sequence Data
Operon
Promoter Regions, Genetic
Restriction Mapping
Temperature

Substances

Bacterial Proteins
DNA, Complementary
GroESL protein, Bacteria
Chaperonins