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. 2001 Mar;7(1):9-14.

Rearrangement of the Myeloid-Lymphoid Leukaemia Gene in Hong Kong Chinese Children With Acute Leukaemia

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  • PMID: 11406670
Free article

Rearrangement of the Myeloid-Lymphoid Leukaemia Gene in Hong Kong Chinese Children With Acute Leukaemia

B M Lit et al. Hong Kong Med J. .
Free article

Abstract

Objective: To determine the frequency of rearrangement of the myeloid-lymphoid leukaemia gene in acute leukaemia in Hong Kong Chinese children.

Design: Immunophenotyping, cytogenetic, and molecular analysis.

Setting: Regional hospital, Hong Kong.

Participants: Bone marrow or peripheral blood samples were taken from 27 children aged 16 years or younger with acute leukaemia, from September 1995 through February 1998.

Main outcome measures: Gene rearrangement was analysed by Southern blotting of HindIII digestion products of mononuclear cell DNA, followed by hybridisation with the myeloid-lymphoid leukaemia P/S4 probe. Nested reverse transcription-polymerase chain reaction analysis was performed to detect and characterise duplication of the myeloid-lymphoid leukaemia gene.

Results: Only one (4%) of 23 children whose marrow or peripheral blood samples contained adequate material for genetic study showed rearrangement in the myeloid-lymphoid leukaemia gene. No children were positive for partial tandem duplication of the myeloid-lymphoid leukaemia gene.

Conclusion: Myeloid-lymphoid leukaemia gene rearrangement is rare in Hong Kong Chinese children with acute leukaemia.

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