The association of serum lipoprotein(a) levels, apolipoprotein(a) size and (TTTTA)(n) polymorphism with coronary heart disease

Clin Chim Acta. 2001 Jul 5;309(1):45-51. doi: 10.1016/s0009-8981(01)00500-9.


Background: The association between lipoprotein(a) levels, apolipoprotein(a) size and the (TTTTA)(n) polymorphism which is located in the 5' non-coding region of the apo(a) gene was studied in 263 patients with severe coronary heart disease and 97 healthy subjects.

Methods: Lp(a) levels were measured by ELISA, apo(a) isoform size was determined by SDS-agarose gel electrophoresis, and analysis of the (TTTTA)(n) was carried out by PCR. For statistical calculation, both groups were divided into low (at least one apo(a) isoform with < or = 22 Kringle IV) and high (both isoforms with >22 KIV) apo(a) isoform sizes, and into low number (<10 in both alleles) and high number of (> or =10 at least one allele) TTTTA repeats.

Results: Lp(a) levels were higher (P=0.007), apo(a) isoforms size < or =22 KIV and TTTTA repeats > or = 10 were more frequent (P=0.007 and 0.01) in cases than in controls. Lp(a) levels were found to be increased with low apo(a) weight in both groups (both P<0.0001). In multivariate logistic regression analysis, only the Lp(a) levels (P=0.005) and (TTTTA)(n) polymorphism (P=0.002) were found to be significantly associated with CHD.

Conclusion: Nevertheless, these results indicate that in CHD patients the (TTTTA)(n) polymorphism has an effect on Lp(a) levels which is independent of the apo(a) size.

Publication types

  • Clinical Trial
  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Apolipoproteins / blood*
  • Apolipoproteins / chemistry
  • Apolipoproteins / genetics*
  • Apoprotein(a)
  • Coronary Disease / blood*
  • Coronary Disease / genetics*
  • Female
  • Humans
  • Lipoprotein(a) / blood*
  • Lipoprotein(a) / chemistry
  • Lipoprotein(a) / genetics*
  • Male
  • Microsatellite Repeats / genetics
  • Middle Aged
  • Multivariate Analysis
  • Polymorphism, Genetic / genetics*
  • Protein Isoforms / chemistry
  • Reference Values


  • Apolipoproteins
  • Lipoprotein(a)
  • Protein Isoforms
  • Apoprotein(a)