Cloning and Characterization of Two Arabidopsis Genes That Belong to the RAD21/REC8 Family of Chromosome Cohesin Proteins

Gene. 2001 Jun 13;271(1):99-108. doi: 10.1016/s0378-1119(01)00499-1.

Abstract

Sister chromatid cohesion is required for proper chromosome segregation during cell division. One group of proteins that is essential for sister chromatid cohesion during mitosis and meiosis is the RAD21/REC8 family of cohesin proteins. Two cohesin proteins are found in yeast; one that functions mainly in mitosis while the other participates in meiosis. In contrast, only one cohesin gene appears to be present in Drosophila. In previous studies we identified an Arabidopsis cohesin protein that is required for meiosis. In this report we describe the isolation and characterization of two additional Arabidopsis cohesin genes. The structure of the genes suggests that they arose via a gene duplication event followed by extensive sequence evolution. Transcripts for the two genes are present throughout the plant and are highest in regions of active cell division, suggesting that the proteins may participate in chromosome cohesion during mitosis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Cell Cycle Proteins / genetics*
  • Chromosomal Proteins, Non-Histone
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Drosophila Proteins*
  • Fungal Proteins
  • Gene Expression Regulation, Plant
  • Genetic Complementation Test
  • In Situ Hybridization
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / genetics*
  • Phosphoproteins / genetics*
  • Phylogeny
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • Arabidopsis Proteins
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • DNA, Complementary
  • Drosophila Proteins
  • Fungal Proteins
  • MCD1 protein, S cerevisiae
  • Nuclear Proteins
  • Phosphoproteins
  • RNA, Messenger
  • SYN2 protein, Arabidopsis
  • SYN3 protein, Arabidopsis
  • Saccharomyces cerevisiae Proteins
  • cohesins
  • vtd protein, Drosophila

Associated data

  • GENBANK/AF281154
  • GENBANK/AF281155