Molecular identification and characterization of the Arabidopsis AtADF1, AtADFS and AtADF6 genes

Plant Mol Biol. 2001 Mar;45(5):517-27. doi: 10.1023/a:1010687911374.

Abstract

Actin depolymerizing factor (ADF) is a key regulator of the organization of the actin cytoskeleton during various cellular activities. We found that ADF genes in Arabidopsis form a large family consisting of at least nine members, four of which were cloned and sequenced in this study. Comparison of genomic and cDNA sequences showed that the AtADF1, AtADF5, and AtADF6 genes all contain two introns at conserved positions. Analysis of transgenic Arabidopsis plants carrying promoter-GUS fusion constructs revealed that AtADF1 and AtADF6 are expressed in the vascular tissues of all organs, whereas expression of AtADF5 is restricted to the root tip meristem. GFP-AtADFI, GFP-AtADF5, and GFP-AtADF6 fusion proteins were found to bind to actin filaments in vivo, and to reorganize the actin cytoskeleton when transiently expressed in plant cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Actin Depolymerizing Factors
  • Actins / metabolism*
  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Blotting, Northern
  • Cloning, Molecular
  • Cytoskeletal Proteins / genetics*
  • Cytoskeletal Proteins / metabolism
  • Destrin
  • Expressed Sequence Tags
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Green Fluorescent Proteins
  • Introns
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Microfilament Proteins / genetics*
  • Microfilament Proteins / metabolism
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Plant Structures / anatomy & histology
  • Plant Structures / metabolism
  • RNA, Messenger / analysis
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment

Substances

  • Actin Depolymerizing Factors
  • Actins
  • Cytoskeletal Proteins
  • Destrin
  • Luminescent Proteins
  • Microfilament Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Glucuronidase

Associated data

  • GENBANK/AF102173
  • GENBANK/AF102825
  • GENBANK/AF183576