Eotaxin-3 but not eotaxin gene expression is upregulated in asthmatics 24 hours after allergen challenge

Am J Respir Cell Mol Biol. 2001 Jun;24(6):682-7. doi: 10.1165/ajrcmb.24.6.4301.


Eotaxin is an important mediator of eosinophil recruitment and activation in the airways of asthmatics. Eotaxin-2 and eotaxin-3 are two recently identified chemokines with activity similar to that of eotaxin. Using quantitative polymerase chain reaction analysis, we determined the messenger RNA (mRNA) expression of eotaxin, eotaxin-2, and eotaxin-3 relative to GAPDH mRNA expression in bronchial biopsies and bronchoalveolar lavage fluid (BALF) cells obtained from subjects with mild asthma, asthmatic subjects 24 h after allergen challenge, and normal control subjects. In bronchial biopsies, gene expression was upregulated in asthmatic subjects as compared with control subjects for eotaxin (log median values 3.18 pg/microg, 95% confidence interval [CI]; 2.27 to 3.79 versus 4.37 pg/microg, 95% CI; 3.97 to 4.65, P = 0.003) and for eotaxin-2 (0.82 pg/microg, 95% CI; 0.08 to 1.72 versus 2.97 pg/microg, 95% CI; 1.97 to 3.45, P = 0.006), but no further increase was observed after allergen challenge. In contrast, eotaxin-3 mRNA expression was not increased in asthmatic compared with control subjects, but was dramatically enhanced 24 h after challenge (median log value 1.93 pg/microg, 95% CI; 0.74 to 3.92 versus 4.62 pg/microg, 95% CI; 3.05 to 6.23, P = 0.036). No significant difference between groups was observed in BALF cell gene expression for any of the chemokines examined. These data suggest that eotaxin-3 rather than eotaxin or eotaxin-2 may account for the ongoing eosinophil recruitment to asthmatic airways in the later stages (24 h) following allergen challenge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Allergens / immunology*
  • Asthma / immunology*
  • Chemokine CCL11
  • Chemokine CCL24
  • Chemokine CCL26
  • Chemokines, CC / biosynthesis
  • Chemokines, CC / genetics*
  • Chemotactic Factors, Eosinophil / biosynthesis
  • Chemotactic Factors, Eosinophil / genetics*
  • Cytokines / biosynthesis
  • Cytokines / genetics*
  • Female
  • Humans
  • Male
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Up-Regulation


  • Allergens
  • CCL11 protein, human
  • CCL24 protein, human
  • CCL26 protein, human
  • Chemokine CCL11
  • Chemokine CCL24
  • Chemokine CCL26
  • Chemokines, CC
  • Chemotactic Factors, Eosinophil
  • Cytokines
  • RNA, Messenger