Helicobacter mustelae lipid A structure differs from that of Helicobacter pylori

FEBS Lett. 2001 Jun 15;499(1-2):1-5. doi: 10.1016/s0014-5793(01)02496-6.

Abstract

The lipid A structure of the Gram-negative bacterium Helicobacter mustelae, a ferret gastric pathogen responsible for the onset of gastric diseases in its host, was investigated. Two variant lipid A structures were found in the same strain. One structure contained a bisphosphorylated beta-(1-->6)-linked D-glucosamine backbone disaccharide with hydroxytetradecanoic acid in amide linkages. Unlike the structure described for the lipid A of the related human Helicobacter pylori gastric pathogen, which contains a C1 phosphate moiety, this lipid A presented phosphate groups at both the C1 and C4' positions, and contained no octadecanoyl fatty acid, which is present in H. pylori. The second lipid A structure had a different fatty acid composition in that 3-OH C(16) replaced most of the amide-linked 3-OH C(14).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrate Conformation
  • Fatty Acids / analysis
  • Fatty Acids / chemistry
  • Ferrets / microbiology
  • Glucosamine / metabolism
  • Helicobacter / chemistry*
  • Helicobacter pylori / chemistry*
  • Lipid A / chemistry*
  • Lipopolysaccharides / pharmacology
  • Magnetic Resonance Spectroscopy
  • Myristates / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Fatty Acids
  • Lipid A
  • Lipopolysaccharides
  • Myristates
  • Glucosamine