Model of interaction of the IL-1 receptor accessory protein IL-1RAcP with the IL-1beta/IL-1R(I) complex

FEBS Lett. 2001 Jun 15;499(1-2):65-8. doi: 10.1016/s0014-5793(01)02515-7.

Abstract

A preliminary model has been calculated for the activating interaction of the interleukin 1 receptor (IL-1R) accessory protein IL-1RAcP with the ligand/receptor complex IL-1beta/IL-1R(I). First, IL-1RAcP was modeled on the crystal structure of IL-1R(I) bound to IL-1beta. Then, the IL-1RAcP model was docked using specific programs to the crystal structure of the IL-1beta/IL-1R(I) complex. Two types of models were predicted, with comparable probability. Experimental data obtained with the use of IL-1beta peptides and antibodies, and with mutated IL-1beta proteins, support the BACK model, in which IL-1RAcP establishes contacts with the back of IL-1R(I) wrapped around IL-1beta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antibody Specificity
  • Cell Line
  • Crystallography, X-Ray
  • Humans
  • Interleukin-1 / chemistry*
  • Interleukin-1 / genetics
  • Interleukin-1 / immunology
  • Interleukin-1 / metabolism*
  • Interleukin-1 Receptor Accessory Protein
  • Mice
  • Models, Molecular
  • Mutation / genetics
  • Protein Binding
  • Protein Conformation
  • Proteins / chemistry*
  • Proteins / metabolism*
  • Rabbits
  • Receptors, Interleukin-1 / chemistry*
  • Receptors, Interleukin-1 / metabolism*
  • T-Lymphocytes
  • Thymus Gland / cytology

Substances

  • Antibodies
  • IL1RAP protein, human
  • Il1rap protein, mouse
  • Interleukin-1
  • Interleukin-1 Receptor Accessory Protein
  • Proteins
  • Receptors, Interleukin-1