The TES gene at 7q31.1 is methylated in tumours and encodes a novel growth-suppressing LIM domain protein

Oncogene. 2001 May 17;20(22):2844-53. doi: 10.1038/sj.onc.1204433.


Many studies suggest that a multi-tissue tumour suppressor gene is located at human chromosome 7q31.1. We have cloned and characterized a novel gene at this locus. The TES gene lies within the minimal region of overlap of several LOH studies and appears to possess the properties of a tumour suppressor. TES is widely expressed and is predicted to encode a protein of 421 amino acids, with three C-terminal LIM domains. Mutation analysis of the coding TES exons in 21 human tumour-derived cell lines revealed the presence of a frameshift mutation in one allele in the breast cancer cell line ZR-75. Methylation of the CpG island at the 5' end of TES appears to be a remarkably frequent finding, occurring in seven out of 10 ovarian carcinomas and in each of the 30 tumour-derived cell lines tested. Moreover, forced expression of TES in HeLa or OVCAR5 cells, resulted in a profound reduction in growth potential, as determined by the colony formation assay. We believe that TES is a tumour suppressor gene that is inactivated primarily by transcriptional silencing resulting from CpG island methylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Breast Neoplasms / genetics
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7 / genetics*
  • CpG Islands
  • Cytoskeletal Proteins
  • DNA Methylation
  • Female
  • Frameshift Mutation
  • Genes, Tumor Suppressor / genetics*
  • HeLa Cells
  • Homeodomain Proteins / genetics*
  • Humans
  • LIM Domain Proteins
  • Loss of Heterozygosity
  • Male
  • Molecular Sequence Data
  • Neoplasms / genetics*
  • Ovarian Neoplasms / genetics
  • Pancreatic Neoplasms / genetics
  • Prostatic Neoplasms / genetics
  • RNA-Binding Proteins
  • Sequence Analysis, DNA
  • Tumor Cells, Cultured
  • Tumor Stem Cell Assay
  • Tumor Suppressor Proteins*
  • Urinary Bladder Neoplasms / genetics


  • Cytoskeletal Proteins
  • Homeodomain Proteins
  • LIM Domain Proteins
  • RNA-Binding Proteins
  • TES protein, human
  • Tumor Suppressor Proteins

Associated data

  • GENBANK/AJ250865